No, they were two separate people. Sir John Hampden (died 1553) lived at Great Hampden in Buckinghamshire and is buried there. He had three daughters but no sons. He bequeathed the bulk of his estate to a distant relative, also called John Hampden, who was,the great grandfather of John Hampden MP “The Patriot”. It is common to find postings on the internet that confuse or merge the identities of the two men.